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pas reagent kit  (Beyotime)


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    Structured Review

    Beyotime pas reagent kit
    Effect of MCL on airway hyperresponsiveness, airway inflammation, and airway mucus hypersecretion in asthmatic mice. (A) Penh values (an indicator of airway responsiveness) of mice after stimulation with different doses of methacholine across different groups. (B) Counts and classification of inflammatory cells across different groups. (C) Percentage of eosinophils among total inflammatory cells in bronchoalveolar lavage fluid (BALF) across different groups. (D) <t>HE</t> <t>staining</t> of lung tissues across different groups (200 × magnification). (E) Peri-bronchial inflammation scores based on HE staining across different groups. (F) <t>PAS</t> staining of lung tissues in each group (200 × magnification). (G) PAS-positive goblet cell score across different groups based on PAS staining. The Control group received normal saline and DMSO. The OVA group was the asthma model group. The OVA + MCL12.5, OVA + MCL25, and OVA + MCL50 groups were treated with 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of MCL in OVA-induced asthmatic mice, respectively. Data are presented as mean ± standard deviation from 6 independent experiments. Data in A and B were analyzed using two-way ANOVA; data in C, E and G are analyzed using one-way ANOVA. Compared with the Control group: ## p < 0.01; Compared with the OVA group: ∗ p < 0.05, ∗∗ p < 0.01.
    Pas Reagent Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/periodic+acid+schiff+(pas)+reagent/Periodic+Acid-Schiff+Staining+Kit/pmc12296505-58-10-13
    Average 99 stars, based on 313 article reviews
    pas reagent kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Protective effect of micheliolide against inflammation and oxidative stress in asthma through the MAPK/NF-κB signalling pathway ☆ "

    Article Title: Protective effect of micheliolide against inflammation and oxidative stress in asthma through the MAPK/NF-κB signalling pathway ☆

    Journal: The World Allergy Organization Journal

    doi: 10.1016/j.waojou.2025.101091

    Effect of MCL on airway hyperresponsiveness, airway inflammation, and airway mucus hypersecretion in asthmatic mice. (A) Penh values (an indicator of airway responsiveness) of mice after stimulation with different doses of methacholine across different groups. (B) Counts and classification of inflammatory cells across different groups. (C) Percentage of eosinophils among total inflammatory cells in bronchoalveolar lavage fluid (BALF) across different groups. (D) HE staining of lung tissues across different groups (200 × magnification). (E) Peri-bronchial inflammation scores based on HE staining across different groups. (F) PAS staining of lung tissues in each group (200 × magnification). (G) PAS-positive goblet cell score across different groups based on PAS staining. The Control group received normal saline and DMSO. The OVA group was the asthma model group. The OVA + MCL12.5, OVA + MCL25, and OVA + MCL50 groups were treated with 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of MCL in OVA-induced asthmatic mice, respectively. Data are presented as mean ± standard deviation from 6 independent experiments. Data in A and B were analyzed using two-way ANOVA; data in C, E and G are analyzed using one-way ANOVA. Compared with the Control group: ## p < 0.01; Compared with the OVA group: ∗ p < 0.05, ∗∗ p < 0.01.
    Figure Legend Snippet: Effect of MCL on airway hyperresponsiveness, airway inflammation, and airway mucus hypersecretion in asthmatic mice. (A) Penh values (an indicator of airway responsiveness) of mice after stimulation with different doses of methacholine across different groups. (B) Counts and classification of inflammatory cells across different groups. (C) Percentage of eosinophils among total inflammatory cells in bronchoalveolar lavage fluid (BALF) across different groups. (D) HE staining of lung tissues across different groups (200 × magnification). (E) Peri-bronchial inflammation scores based on HE staining across different groups. (F) PAS staining of lung tissues in each group (200 × magnification). (G) PAS-positive goblet cell score across different groups based on PAS staining. The Control group received normal saline and DMSO. The OVA group was the asthma model group. The OVA + MCL12.5, OVA + MCL25, and OVA + MCL50 groups were treated with 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of MCL in OVA-induced asthmatic mice, respectively. Data are presented as mean ± standard deviation from 6 independent experiments. Data in A and B were analyzed using two-way ANOVA; data in C, E and G are analyzed using one-way ANOVA. Compared with the Control group: ## p < 0.01; Compared with the OVA group: ∗ p < 0.05, ∗∗ p < 0.01.

    Techniques Used: Staining, Control, Saline, Standard Deviation

    Related Articles

    Staining:

    Article Title: Effects of Enterotoxigenic Escherichia coli Challenge on Jejunal Morphology and Microbial Community Profiles in Weaned Crossbred Piglets.
    Article Snippet: .. Using a PAS staining reagent kit (C0142M, Beyotime, Shanghai, China), periodic acid–Schiff (PAS) staining was performed according to the protocol. ..

    Article Title: AhR agonist tapinarof ameliorates lupus autoimmunity by suppressing Tfh cell differentiation via regulation of the JAK2‐STAT3 signaling pathway
    Article Snippet: Paraffin blocks were subsequently sectioned at 6 μm thickness on a microtome (Leica Biosystems). .. The sections were stained with hematoxylin and eosin (H&E) using an H&E Staining Kit (Beyotime) or with Periodic‐Acid‐Schiff (PAS) reagent using a PAS Staining Kit (Beyotime), following manufacturers' protocols. ..

    Article Title: AhR agonist tapinarof ameliorates lupus autoimmunity by suppressing Tfh cell differentiation via regulation of the JAK2-STAT3 signaling pathway.
    Article Snippet: Paraffin blocks were subsequently sectioned at 6 μm thickness on a microtome (Leica Biosystems). .. The sections were stained with hematoxylin and eosin (H&E) using an H&E Staining Kit (Beyotime) or with Periodic‐ Acid‐Schiff (PAS) reagent using a PAS Staining Kit (Beyotime), following manufacturers' protocols. ..

    Article Title: Protective effect of micheliolide against inflammation and oxidative stress in asthma through the MAPK/NF-κB signalling pathway
    Article Snippet: The right lung tissue was excised from mice, fixed in 4% paraformaldehyde for 48 h, followed by dehydration, transparency processing, paraffin embedding, sectioning (3 μm thick), deparaffinisation, staining with haematoxylin (Sinopharm, Beijing, China) for 20 min, staining with eosin (Sinopharm) for 1 min, washing with phosphate-buffered saline (PBS), dehydration with ethanol, mounting with neutral resin in a fume hood, and covering with a coverslip. .. The lung tissue sections were subjected to staining with a PAS reagent kit (Beyotime, Shanghai, China). ..

    Article Title: Effects of Enterotoxigenic Escherichia coli Challenge on Jejunal Morphology and Microbial Community Profiles in Weaned Crossbred Piglets
    Article Snippet: .. Using a PAS staining reagent kit (C0142M, Beyotime, Shanghai, China), periodic acid–Schiff (PAS) staining was performed according to the protocol. ..

    Tissue:

    Article Title: Protective effect of micheliolide against inflammation and oxidative stress in asthma through the MAPK/NF-κB signalling pathway
    Article Snippet: The right lung tissue was excised from mice, fixed in 4% paraformaldehyde for 48 h, followed by dehydration, transparency processing, paraffin embedding, sectioning (3 μm thick), deparaffinisation, staining with haematoxylin (Sinopharm, Beijing, China) for 20 min, staining with eosin (Sinopharm) for 1 min, washing with phosphate-buffered saline (PBS), dehydration with ethanol, mounting with neutral resin in a fume hood, and covering with a coverslip. .. The lung tissue sections were subjected to staining with a PAS reagent kit (Beyotime, Shanghai, China). ..



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    Effect of MCL on airway hyperresponsiveness, airway inflammation, and airway mucus hypersecretion in asthmatic mice. (A) Penh values (an indicator of airway responsiveness) of mice after stimulation with different doses of methacholine across different groups. (B) Counts and classification of inflammatory cells across different groups. (C) Percentage of eosinophils among total inflammatory cells in bronchoalveolar lavage fluid (BALF) across different groups. (D) <t>HE</t> <t>staining</t> of lung tissues across different groups (200 × magnification). (E) Peri-bronchial inflammation scores based on HE staining across different groups. (F) <t>PAS</t> staining of lung tissues in each group (200 × magnification). (G) PAS-positive goblet cell score across different groups based on PAS staining. The Control group received normal saline and DMSO. The OVA group was the asthma model group. The OVA + MCL12.5, OVA + MCL25, and OVA + MCL50 groups were treated with 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of MCL in OVA-induced asthmatic mice, respectively. Data are presented as mean ± standard deviation from 6 independent experiments. Data in A and B were analyzed using two-way ANOVA; data in C, E and G are analyzed using one-way ANOVA. Compared with the Control group: ## p < 0.01; Compared with the OVA group: ∗ p < 0.05, ∗∗ p < 0.01.
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    Image Search Results


    Effect of MCL on airway hyperresponsiveness, airway inflammation, and airway mucus hypersecretion in asthmatic mice. (A) Penh values (an indicator of airway responsiveness) of mice after stimulation with different doses of methacholine across different groups. (B) Counts and classification of inflammatory cells across different groups. (C) Percentage of eosinophils among total inflammatory cells in bronchoalveolar lavage fluid (BALF) across different groups. (D) HE staining of lung tissues across different groups (200 × magnification). (E) Peri-bronchial inflammation scores based on HE staining across different groups. (F) PAS staining of lung tissues in each group (200 × magnification). (G) PAS-positive goblet cell score across different groups based on PAS staining. The Control group received normal saline and DMSO. The OVA group was the asthma model group. The OVA + MCL12.5, OVA + MCL25, and OVA + MCL50 groups were treated with 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of MCL in OVA-induced asthmatic mice, respectively. Data are presented as mean ± standard deviation from 6 independent experiments. Data in A and B were analyzed using two-way ANOVA; data in C, E and G are analyzed using one-way ANOVA. Compared with the Control group: ## p < 0.01; Compared with the OVA group: ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: The World Allergy Organization Journal

    Article Title: Protective effect of micheliolide against inflammation and oxidative stress in asthma through the MAPK/NF-κB signalling pathway ☆

    doi: 10.1016/j.waojou.2025.101091

    Figure Lengend Snippet: Effect of MCL on airway hyperresponsiveness, airway inflammation, and airway mucus hypersecretion in asthmatic mice. (A) Penh values (an indicator of airway responsiveness) of mice after stimulation with different doses of methacholine across different groups. (B) Counts and classification of inflammatory cells across different groups. (C) Percentage of eosinophils among total inflammatory cells in bronchoalveolar lavage fluid (BALF) across different groups. (D) HE staining of lung tissues across different groups (200 × magnification). (E) Peri-bronchial inflammation scores based on HE staining across different groups. (F) PAS staining of lung tissues in each group (200 × magnification). (G) PAS-positive goblet cell score across different groups based on PAS staining. The Control group received normal saline and DMSO. The OVA group was the asthma model group. The OVA + MCL12.5, OVA + MCL25, and OVA + MCL50 groups were treated with 12.5 mg/kg, 25 mg/kg, and 50 mg/kg of MCL in OVA-induced asthmatic mice, respectively. Data are presented as mean ± standard deviation from 6 independent experiments. Data in A and B were analyzed using two-way ANOVA; data in C, E and G are analyzed using one-way ANOVA. Compared with the Control group: ## p < 0.01; Compared with the OVA group: ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: The lung tissue sections were subjected to staining with a PAS reagent kit (Beyotime, Shanghai, China).

    Techniques: Staining, Control, Saline, Standard Deviation